Determination of thiol/disulphide homeostasis as a new indicator of oxidative stress in dairy cows with subclinical endometritis

The objective of this study was to determine thiol/disulphide homeostasis (TDH) in infertile cows with subclinical endometritis (SCE). Endometrial cytological samples were collected using a cytobrush diagnose SCE 36 cows. According the results cytology examination, those acute classified as Group I (n = 20) and chronic II 16). A control group formed heifers III 20). Blood taken from each on day diagnosis (day 0) analyse TDH. In both Giemsa method immunocytochemical staining applied inflammation activity status. 55.55% (20/36) endometritis, determined be active, 44.44% (16/36) it had become chronic. native thiol total levels found statistically significantly lower (206.54 ± 8.30 μmol/L; 227.11 9.30 μmol/L) cases (225.15 11.89 247.96 10.80 compared heathy (308.47 13.59 336.83 15.5 μmol/L respectively) (P<0.001). Disulphide levels, disulphide/total thiol, thiol/total disulphide/native ratios similar all groups (P>0.05). diagnostic accuracy which can used SCE, 92.8%, that 89.3% disulphide 64.3% according ROC curve analysis. These demonstrate TDH is reliable sensitive indicator oxidative stress cow abnormal might play role pathogenic mechanisms. This first evaluate dairy new stress.